Application

Western blot: 1-10 µg/mL using ECL. An antibody raised against this peptide has detected a glycosylated band at ~62 kDa in membranes from rat inner medulla (Nonoguchi et al. 1995). N-Glycanase reduced the size to ~36 kDa (deglycosylated form).

Immunohistochemistry: 2-10 µg/mL on paraformaldehyde fixed tissue. AVP-V2 has been localized in terminal IMCD and other areas of rat kidney (Nonoguchi et al. 1995).

ELISA: 0.5-1.0 µg/mL using 1 µg/mL of control peptide to coat plate.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Arginine Vasopressin Receptor V2 Antibody is an antibody against Arginine Vasopressin Receptor V2 for use in ELISA, IH & WB.

Research Sub CategoryCNS Control of MetabolismHormones & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

21 amino acid peptide from the 3rd cytoplasmic domain of rat AVP-V2 (Lolait et al. 1992; Fisov et al. 1994).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB3512P

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Specificity

Arginine vasopressin receptor (AVP-V2), rat. The peptide used for immunization has no significant similarity with AVP V1a or V1b receptors.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.